Western blot analysis of skeletal muscle GLUT4 levels relative to β-actin revealed significant increases in all the diabetic rats supplemented with 5%, 7.5% or 15% RBBO compared to the untreated DM rats (ranging from 51% for DMRH to 96% for DMRL) whereas a low level of GLUT 4 expression was found in DM-MET and untreated DM rats as shown in Figure 1. It should be noted that the greatest amount of GLUT 4 protein expression was observed in both normal rats and especially in the diabetic rats supplemented with 5% RBBO (DMRL) as shown in Figure 1.