The enzyme solutions were mixed with different organic solvents, ethanol, methanol, octane, hexane, toluene, actetonitrile,
benzene, dimethyl sulfoxide and acetone, to yield a desired final concentration (v/v 25%). The enzyme samples
were exposed to solvent at 30°C with shaking at 150 rpm for 48 h, after which their residual activity was measured
using p-NPP method. The initial lipase activity (without containing organic solvents) was considered to be 100%.
The enzyme solutions were mixed with different organic solvents, ethanol, methanol, octane, hexane, toluene, actetonitrile,benzene, dimethyl sulfoxide and acetone, to yield a desired final concentration (v/v 25%). The enzyme sampleswere exposed to solvent at 30°C with shaking at 150 rpm for 48 h, after which their residual activity was measuredusing p-NPP method. The initial lipase activity (without containing organic solvents) was considered to be 100%.
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