Serial dilutions were subsequently prepared with the same diluent. Populations of B. animals were enumerated by the pour plating technique using 1 mLof each dilution in MRS-LP agar (oxoid. UK) followed anaerobic incubation (Anaerobic system by Anaerogen. BBL EUA) at 43 °C for 72 h. The results were expressed as log CFU/g and also as survival rate according to Magarinos. ไม่ต้องSelaive. Costa, Flores, and Pizarro (2007).