A contact angle of water (model Oca20, dataphysics instruments) was used to measure the contact angle of water in air on the surface of chitosan/glycerol films. A film sample (20 mm × 80 mm) was put on a movable sample stage and levelled horizontally; then a drop of about 3 μL of distilled water was placed on the surface of the film using a microsyringe. The contact angle was measured in a conditioned room by recording contact angle values. Image analyses were carried out using SCA20 software. Ten replicates were made per formulation.