Briefly, 100 L of 500 nM fluoresceinand 25 L of diluted M. plumbea extracts were pipetted into each working well of a microplate (96 well) preincubated at 37 ◦C.
Thereafter, 25 L of 250 mM AAPH was quickly added and the microplate was shaken for 5 s in a microtiter plate fluorometer
Multiskan Ascent instrument (Labsystems, Helsinki, Finland).