to 9 mL of sterile PBS and left to hydrate and dissolve under constant agitation in an orbital incubator at 37 C for 1 h. In both cases,the resulting solutions were subjected to serial dilutions using phosphate buffer saline. Each dilution was pour plated on a MRS agar (MRS Agar, Oxoid Ltd., Basingstoke, UK) and the plates were stored at 37 C for 72 h under anaerobic conditions to allow colonies to grow. Enumeration of the bacteria was performed in triplicate following the standard plating methodology
(Champagne, Ross, Saarela, Hansen, & Charalampopoulos, 2011)and the total counts of the viable bacteria were expressed as log colony forming units per gram (log CFU/g).
to 9 mL of sterile PBS and left to hydrate and dissolve under constant agitation in an orbital incubator at 37 C for 1 h. In both cases,the resulting solutions were subjected to serial dilutions using phosphate buffer saline. Each dilution was pour plated on a MRS agar (MRS Agar, Oxoid Ltd., Basingstoke, UK) and the plates were stored at 37 C for 72 h under anaerobic conditions to allow colonies to grow. Enumeration of the bacteria was performed in triplicate following the standard plating methodology(Champagne, Ross, Saarela, Hansen, & Charalampopoulos, 2011)and the total counts of the viable bacteria were expressed as log colony forming units per gram (log CFU/g).
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