The microbiological analyses were as follows: a total amount of 10 g of fish (after removal of the film), from at least 3 different packages,was collected and placed in a sterile plastic bag .with 90 mL of buffered 0.1% peptone water
(Oxoid, Basingstoke, UK) in a vertical laminar-flow cabinet (mod.
AV 30/70 Telstar, Madrid, Spain.) After 1 min in a Stomacher
blender (model Colworth Stomacher 400), appropriate dilutions
were prepared for the following microorganisms determinations:
(i) total bacteria counts (TBC) on spread plates of Iron Agar 1% NaCl
incubated at 15 C for 72 h