Those explants that had formed somatic embryos were transferred to somatic
embryo germination and regeneration medium, which contained MS salts, B5
vitamins, 3%
sucrose, 0.8% agar, and 4 mg/L 6-benzyl aminopurine (6-BAP). Tissue culture conditions
were as described in the previous paragraph. After 8 weeks, the percentage of plants that had
regenerated from the somatic embryos was determined according to the following equation: