Liquid a-amylase from Bacillus licheniformis
(500 U/mg protein, 10 mg protein/mL, Sigma Aldrich, USA) and
amyloglucosidase from Aspergillus niger (P300 U/mL, Sigma
Aldrich, USA) were diluted in acetate buffer (pH 5, 0.2 M) to obtain
the stock solutions of 450 U/mL a-amylase and 5.25 U/mL amyloglucosidase,
respectively.