The larval microbiota was analysed using a Biostep TV400 DGGE
vertical electrophoresis system with 16.5 17.5 cm gels. PCR
products were run on an 8% (w/v in 1 TAE) polyacrylamide gel
containing a 40e60% gradient of denaturing agent (100% denaturant
contains 7 M urea an 40% w/v formamide) at 60 C for 15 h at
100 V. Gels were stained with 0.11 M silver nitrate for band visualization
[46].