To monitor the menstrual cycle, we performed daily virginal
smears as previously described.Each phase of menstrual
cycle is characterized by superficial nucleated cells for proestrus,
superficial cytode cells for estrus, superficial nuclearted cell plus
luekocyte for metestrus, and flourish leukocyte for anestrus,
respectively. At the end of the 6-week intensive treadmill
training, ovary epithelial cells changes were obvious as an
outcome. Rats in group E showed longer duration of anestrus
phase with a large number of small underlayer cells and delayed
onset of estrus while typical middle layer cells were found in rats
from groups R, G, and O, respectively (Fig. 3).