Each assay
was consisted of 35 PCR cycles. The parameters for each cycle were
1 min of denaturation at 95 C; 2 min of annealing at 65 C for the
first 10 cycles, decrementing to 60 C by cycle 15; and 1.5 min of
elongation at 72 C, incrementing to 2.5 min from cycles 25 to 35