Hydrogen production from crude glycerol by two-stage processes of dark fermentation
using Klebsiella sp. TR17 and photo fermentation using Rhodopseudomonas palustris TN1 (Rps.
palustris TN1) was investigated in batch experiments. In dark fermentation, the cumulative
hydrogen production and hydrogen yield was 64.24 mmol H2/L and 5.74 mmol H2/g COD
consumed, respectively with 80.21% of glycerol conversion rate. The dark fermentation
effluent (DFE) was employed for photo fermentation. Effect of DFE concentrations (0e5
times dilution), with and without supplementation of yeast extract (2.3 g/L) þ NaHCO3
(0.63 g/L), and glutamate (2e8 mM) were optimized. The optimal conditions for hydrogen
production from Rps. palustris TN1 were 5 times dilution of DFE without the supplement of
yeast extract þ NaHCO3, and 2 mM glutamate. Under the optimum conditions, the cumulative
hydrogen production of 3.12 mmol H2/L and hydrogen yield of 0.68 mmol H2/g
COD consumed was obtained. The total hydrogen yield of two-stage processes was estimated
to be 6.42 mmol H2/g COD consumed which was 10.4% of the theoretical yield.
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