Explants with PGR induced multiple shoot initials were fixed in FAA solution (50% ethanol: 40% formalin: acetic acid, 90:5:5 v/v/v) and directly taken to hand sectioning [13]. Dehydration in ethanol series was neglected due to severe level of tissue shrinkage. The fixed tissues were manually sectioned longitudinally using several fine blades and immediately stained with 1% safranin.