In this study developed a direct STR typing protocol for biological samples (blood, semen and saliva) is successful. The result shown that found of presumptive test kits type have impact the DNA typing, including the surface material is different, it can affect the DNA typing as well. For the experiment, the presumptive test of blood that does not affect with the DNA typing included luminol, Hemastic®, Leucomalachite green and Kastle-Meyer respectively.