2.3. Operation of fed-batch strategy
The fed-batch cultivation was started on a batch culture with
0.24 g/L inoculums size and 300 lmol/m2
/s light intensity. When
the initial nitrate was depleted, the 100-fold concentrated nitrate
solution or concentrated fresh medium was then pulse-fed into
the culture to reach a nitrate concentration of 5.0 and 10 mM.
The timing for starting new pulse-feeding was when the nitrate
concentration in the culture broth was nearly depleted. Liquid
samples were collected from the culture broth at set time intervals
to determine the cell concentration, pH, C-PC content and residual
nitrate concentration.