Fig. 1. Overlays of far-UV CD spectra of protein MNEI (panel A) and Y65R (panel B) at different pH values. Protein concentration of 0.3 mg/ml, in 20 mM sodium-phosphate
buffer, has been used for all measurements recorded at 25 C. pH 3.3 (red curve); pH 5.1 (blue curve) and pH 7.0 (green curve). (For interpretation of the references to colour
in this figure legend, the reader is referred to the web version of this article.)