This study indicated that biofuel production were increased (>1.2-folds) by immobilized cell technique. The immobilized S. cerevisiae yielded highest ethanol (11.63 g/L) after 72 h of cultivation (Fig. 2a). The similar trend was also observed by immobilized E. aerogenes while the production of ethanol and hydrogen were also increased to 1.66 g/L and 1,759 mL/L, respectively (Fig. 2b). The comparison between the production of biofuel from free cell suspension and immobilized cell was given in Table 4.