Inour study,the frequency of Brucella spp.inmilk samples was higher than some previous studies. According to Ilhan et al. (2008), Hamdy and Amin (2002) and Abbas and Aldeewan (2009), the prevalence of Brucella spp. was 7.8%, 51.4% and 14.7%, respectively. This difference in Brucella prevalence may be due to the diagnostic approaches used for detection of Brucella spp., type of specimens, the geographical region and etc. According to previous studies, the PCR based assays were detected higher number of positive milk samples than cultural methods.