A pathogenic bacterial cocktail in the respective broth was serially
diluted in PPS, with the final dilution 10−2 done in UHT full-fat cow's
milk. Of this, 1 mL was inoculated into 79 g UHT full-fat cow's milk
that was used to prepare traditional mutandabota, and 1 mL was inoculated
into 78 g UHT full-fat cow's milk that was used to prepare yoba
mutandabota. In both traditional and yoba mutandabota, the initial concentration
of the pathogenic cocktail suspension was approximately
5.5 log cfu/mL. For yoba mutandabota, inoculation of the pathogenic
cocktail suspension was done simultaneously with L. rhamnosus yoba
inoculation (Section 2.1). Independent triplicate experiments were
done with each pathogenic bacterial cocktail. Non inoculated samples
(negative controls inoculated with 1 mL full-fat UHT milk) were also