Thus, fermentation experiments wereperformed in order to achieve high cell density in both TB anddefined media to increase consequently the amount of heterolo-gous peptide produced. The total amount of protein and the amountof Pa-MAP 2 produced per gram of biomass was approximately 0.1-fold higher in TB media compared to fed batch in defined media.Also in TB media, cells were harvested in six hours, while in definedmedia this occurred at ten hours after beginning of cultivation, sug-gesting a higher productivity rate over time associated with theuse of complex media. The yield of Pa-MAP 2 produced using TBmedia was 50-fold higher than the antimicrobial peptide HD4also expressed in TB media using an ELP-intein system (Shen et al.,2010).