It was noted that L. casei Shirota converted both glucose and fructose to LA, and it began to utilize fructose when glucose was about to be depleted. Compared to the fermentation using defined medium as feedstock, more cell biomass (6.54 g L−1) was produced in the fermentation using bakery waste hydrolysate, leading to a higher glucose uptake rate (4.14 g L−1 h−1) and productivity of LA