Each well received 25 mL 0.1 M TriseHCl buffer pH 7.6
containing 0.15 M NaCl and 5 mM CaCl2 and 5 mM MgCl2. A 100 mL
aliquot of supernatant was added to the first well of the column.
Subsequently, 200 mL suspension of 2% rabbit erythrocytes suspension
containing 0.15 M NaCl was added to each well.