Mice (4 and 6 weeks old)
were vaccinated with 1 (day 0) or 2 (days 0 and 7) doses via
various dilutions of PRV, SAD, or LEP vaccines and various
administration routes, as specified. Mice were administered 0.5 mL of vaccine by the IP route, and the equivalent vaccine dose (diluted one-fifth the dilution of the IP vaccine) was administeredIM in the thigh or hip region and SC in the neck region by use of 0.1 mL, because a 0.5-mL volume was too great for these routes. The challenge in all experiments was IC1 at the times specified after vaccination. Serum collection and as