Biofilms are surface-associated, three-dimensional multicellular structures whose integrity depends upon the extracellular matrix produced by their constituent bacterial cells. Biofilm formation occurs as a result of a sequence of events: adhesion of individual microbial cells to a surface, cell proliferation and aggregation into microcolonies, matrix production, and cell detachment. Initiation of biofilm formation is characterized by the interaction of bacterial cells with a surface and with each other. The biofilm matures through the production of extracellular matrix which is mainly composed of sticky extracellular polysaccharides (EPS) and proteins