2.10. TRAP assay
The luminol-enhanced chemiluminescence (CL) was used to
monitor the peroxyl radical reaction as described previously
(Cˇ ízˇová, Lojek, Kubala, & Cˇ ízˇ, 2004). The CL signal is driven by the
production of luminol derived radicals from thermal decomposition
of AAPH. The TRAP value was determined from the duration