To prepare a fresh culture of the bacteria, inoculate the bacteria
onto Petri dishes (9-cm diameter) containing 172b agar
medium
Incubate the Petri dishes at 28°C for 7 days.
Inoculate a 250-mL Erlenmeyer fl ask containing 30 mL of
172b liquid medium with the fresh culture ( see Note 7).
Incubate the flask at 28°C on a rotary shaker at 250 rpm for
96 h.