The protein samples (1.0 mL of 4.0 mg of protein/mL) were dissolved in 1.0 mL of the sample buffer (4% SDS, 20% glycerol,10% bmercaptoethanol,and 0.125 M TriseHCl, pH 6.8), heated to 100 C for 3 min, and centrifuged at 1800 g to remove any particulates.
The protein samples (1.0 mL of 4.0 mg of protein/mL) were dissolved in 1.0 mL of the sample buffer (4% SDS, 20% glycerol,10% bmercaptoethanol,and 0.125 M TriseHCl, pH 6.8), heated to 100 C for 3 min, and centrifuged at 1800 g to remove any particulates.
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