. Hydrogen peroxide injury model
HSF cells were laid on 96-well plate with 1 × 104 cells per well.
After 24 h of culture, hydrogen peroxide was added respectively to
final concentrations of 0.5, 1.5, 2.0, 2.5 and 3.0 mmol/L. No hydrogen
peroxide was added into blank control group. Six parallel wells
were set for every group. Twelve hours later, cells were treated by
MTT for 4 h. Then, cell viability was determined by Gen5 ELIASA
(Bio-Rad Co., U.S.).