Nine strains were able to produce detectable lipase activity in SSF, and the highest activity was observed for Thermomyces lanuginosus and R. pusillus . Only T. indicae seudaticae N31 and R. pusillus produced lipase in the both SSF and SmF systems.
Since T. indicae seudaticae N31 has not been previously described for lipase production, it was selected for subsequent studies of production and characterization of this enzyme.