Computers just like microbes are ubiquitous and continue to have an increased presence in everyday lives. The aim of this study was to investigate and compare the level, types and antibiotic susceptibility of bacterial contaminants of keyboards and mice in general offices and internet cafés. A total of 100 samples were collected by swabbing the surfaces of keyboards and mice. Serial dilution and aerobic colony counting was performed by pour plating on PCA (Plate Count Agar) for each sample. Isolates were identified by standard biochemical methods whilst the disc diffusion technique was applied in Antibiotic Susceptibility Testing of each bacterial isolate. All surfaces of keyboard and mice were contaminated (>105) with means of 5.664×108 and 1.305×107 CFU for office keyboard and mice respectively and 1.52×109 and 1.901×107 CFU for café keyboard and mice respectively. There was a 48.02% contamination difference between office and cafés which was significant; keyboards (P=0.000) and mice (P=0.001). Eight bacteria species were isolated with the highest B. cereus, 70(47.95%) and the least E. coli 3(2.05%). Majority of the contaminants were pathogenic 117(80.41%) made up of 42.47% from café samples and 37.94% from office. S.
pneumonia, Enterococcus spp. and Coagulase Negative Staphylococcus were the least susceptible organisms (25%) whilst Ampicillin was the least effective antibiotic (0%). These findings suggest high contamination of keyboard and mice with pathogenic bacteria and associated potential of transmission and infection.