A total of four actinomycetes were used in this study to check their antagonistic effect on seed and soil borne plant pathogens, that is, Fusarium oxysporum (wilt in tomato), Rhizoctonia solani (sheath blight in maize), Colletotrichum tranctum (Anthracnose in soyabean), Colletotrichum capsici (Anthracnose in chilli) and Helminthosporium oryzae (brown spot in rice) by in vitro dual-culture assay using the modified method of Cherif and Benhamou (1990).