Specific amplification of 16s rRNA was preformed using 16s rRNA Gold primers set
(name of primer Forward: QUGP-F4-CCGCCTGGGGAGTACG and Name of primer
Reverse: QUGP-Rn2- TGACGGGCGGTGTGTACAAG) as per Barghouthi (2011). The
primers were custom synthesized through facility of Integrated DNA Technologies,