The efficacy of the separation is assessed by thin-layer chromatography (TLC). Glass plates coated with silica gel, 0.25 mm layer, and a mixture of light petroleum, diethyl ether and acetic acid 70:30:2 (V/V/V) as developing solvent are used. After plate development and solvent evaporation, the plate is sprayed with a phosphomolybdic acid solution and heated at 120-130ºC to visualise spots. As can be observed in Figure 1, an efficient separation is easily checked by confirming that unoxidized TG appear only in the first fraction and the spots of higher polarity can only be seen in the second fraction.