The SBF was removed after several periods of immersion and silicon, calcium and phosphorus were determined in the removed SBF by inductively coupled plasma optical emission spectrometry (ICP-OES Perkin-Elmer Optima 2000™). For TEM study the samples were prepared by careful removal of the reaction layer from sample surfaces using a razor blade, and dispersing the powder on the surface of ethanol in a Petri dish. The powder specimens after drying were then collected on carbon coated TEM copper grids of 300 mesh. Electron beam transparent particles were chosen for TEM examination by selected area diffraction (SAD) and also EDS.