in the absence of unique restriction sites, alternative methods including DiSec/TriSec [25], which allows the ganaration ofapcified sticky-ends, may be used or a retriction-independent method like the overlapping primer method should suffice. the method has proven to be invaluable in deciphering the involvement of parasite-specific inserts in structure-activity relationships of PfAdoMetDC/ODC and PfPdxK (manuscript in preparation).