It is necessary to point out that if the UV or fluorescence spectra of
the analytes were sufficiently different, the chromatograms could be
processed in the total time range. However, in multicomponent systems
it is very likely to find spectral similarities among analytes. In this situation, if the full chromatograms were processed, unsuitable results
would be obtained because the mathematical pseudorank would be
smaller than the chemical rank [12]. As will be seen below, to overcome
this situation, MCR-ALS was applied in selected time ranges, ensuring
that each partial chromatographic region includes analytes with different spectral profiles.
It is necessary to point out that if the UV or fluorescence spectra ofthe analytes were sufficiently different, the chromatograms could beprocessed in the total time range. However, in multicomponent systemsit is very likely to find spectral similarities among analytes. In this situation, if the full chromatograms were processed, unsuitable resultswould be obtained because the mathematical pseudorank would besmaller than the chemical rank [12]. As will be seen below, to overcomethis situation, MCR-ALS was applied in selected time ranges, ensuringthat each partial chromatographic region includes analytes with different spectral profiles.
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