were tested for reactivity with: Toxoplasma gondii antigen by IFA as described previously Baneth et al., 1996b., with Neospora caninum using a commercial IFA antigen VMRD, Pullman, WA., with Leishmania donoÍani antigen by enzyme-linked immunosorbent assay ELISA. as described by Jaffe et al. 1988. and with Ehrlichia canis antigen using a commercial dot–blot ELISA Immunocomb; Biogal.. No seroreactivity was found against any of these 4 organisms. These results verify the specificity of the IFA test for H. canis.