The second synthetic dye solution which is more similar to real textile industry wastewater was used. The composition of the solution was 12 g NaCl, 0.049 Na_3 PO_4∙12H_2 O , 0.024 g NaNO_3, 0.255 g KHSO_4 , 200 mg Reactive Black 5 dye, 200 mg Procion Red MX-5B, the soap and the washing solution from cotton, and 1 g of yeast for 1 L solution.