Photon correlation spectroscopy (PCS) with a Malvern zetasizer ZS (Malvern Instruments, UK) was used to determine the particle size, profile the size distribution (polydispersity index, PI) and zeta potential of the nanoparticles. Briefly, the Zeta potential and the poly dispersity index (PDI) of the nanoparticle formulations were determined using the Zetasizer (Nano ZA, Malvern Instruments, UK). In this method the sample was measured at 25 ◦ C with an angle detection of 90◦. The concentration of the samples for analysis on the Zeta Sizer was 20–400 kilo counts per second (KCPS) and the intensity of diffraction was 100000 counts per second.