All the inoculated solid media, after 72 h of
aerobically cultivation at 25 ºC showed single,
morphologically well-formed colonies. Isolated
representatives of the dominant colonies were spread
on plates containing WWM, MLM and B4 solid media.
The plates were aerobically incubated at 25 ºC for 30 d
and checked periodically for the presence or absence
of crystals using optical microscopy. Isolated
representatives of the dominant colony morphologies
with carbonate-forming capacity in B4 medium
(seventeen major colony types) were selected and
purified, by restreaking them twice. All the experiments