The production of spores of Beauveria bassiana LPB-01 by solid-state fermentation by utilizing refused potatoes, as substrate/support was found viable. The results employing different agro-industrial residues (refused potatoes, coffee husk, refused potatoes + sugar-cane bagasse 50:50, and refused potatoes + coffee husk 50:50) demonstrated that the best substrate/support was the mixture of refused potatoes and sugar-cane bagasse, resulting 3.8x107 spores.g-1 of dry matter. The analysis of the quantity of refused potatoes and sugar-cane bagasse showed that a higher spore production (3.4x109 spores.g-1 of dry matter) was obtained with the proportion of 60:40%, respectively.
The studies on the effect of physical parameters of the process in Erlenmeyer flasks demonstrated that the best conditions for spore production were temperature of 26º C, pH 6.0, and initial moisture content of the substrate/support 75% and inoculation rate of 107 spores.g-1 of dry matter. In column type bioreactors best result of spore production was 9.9x109 spores.g-1 of dry matter and was achieved with optimized conditions of initial moisture content (65%) and aeration rate (60 ml. min -1). Finally, a kinetic study of spore production with optimized conditions, concomitant with a respirometric analysis during the development of the fungus showed a correlation between the spore production and the total O2 consumption.
The production of spores of Beauveria bassiana LPB-01 by solid-state fermentation by utilizing refused potatoes, as substrate/support was found viable. The results employing different agro-industrial residues (refused potatoes, coffee husk, refused potatoes + sugar-cane bagasse 50:50, and refused potatoes + coffee husk 50:50) demonstrated that the best substrate/support was the mixture of refused potatoes and sugar-cane bagasse, resulting 3.8x107 spores.g-1 of dry matter. The analysis of the quantity of refused potatoes and sugar-cane bagasse showed that a higher spore production (3.4x109 spores.g-1 of dry matter) was obtained with the proportion of 60:40%, respectively.The studies on the effect of physical parameters of the process in Erlenmeyer flasks demonstrated that the best conditions for spore production were temperature of 26º C, pH 6.0, and initial moisture content of the substrate/support 75% and inoculation rate of 107 spores.g-1 of dry matter. In column type bioreactors best result of spore production was 9.9x109 spores.g-1 of dry matter and was achieved with optimized conditions of initial moisture content (65%) and aeration rate (60 ml. min -1). Finally, a kinetic study of spore production with optimized conditions, concomitant with a respirometric analysis during the development of the fungus showed a correlation between the spore production and the total O2 consumption.
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