The following reagents are used for estimation of amylose content and for preparation of standard:
95% Ethanol: Prepared from 100% Ethanol
1N NaOH, Iodine - Potassium iodide solution
Standard amylose: Obtained from HIMEDIA
1N Acetic acid: From NICE Chemicals
Glassware: Borosil
Water bath: High Precision water bath from Acmas was used
Spectrophotometer: From Amersham Biosciences to measure OD
Cuvette: Quartz Cuvette to measure OD
Software: HandyGraph Software to draw graph
1N NaOH solution: Dissolve 40g of NaOH in 1000ml distilled water
1N Acetic acid solution: Dilute 57.5 ml glacial acetic acid to 1000ml using distilled water
Iodine - Potassium iodide solution: Dissolve 0.26 g of Iodine in 10 ml of Potassium iodide solution containing 2.6 g
of KI