In this study, the detrimental effect of ethylene was most likely to be the cause of leaf senescence on shoots of C. hystrix since AgNO3 had effectively overcome the senescence. AgNO3, cobalt chloride, silver thiosulphate and aminoethoxyvinylglycine are among the ethylene inhibitors that have been widely supplemented in tissue culture media to reduce the detrimental effects of ethylene the production of ethylene and its effect is highly genotype-dependent. The regeneration of Capsicum annuum and Corymbia maculata was also improved through the addition of Ag? in the culture medium