A laboratory scale batch culture in filtered shrimp wastewater with macroalgae fresh weight of 1.0 kg m3 was performed. Culture medium was prepared and macroalgae were placed in the shrimp wastewater which is the culture medium. Each transparent of 1.0 L capacity glass bottle was stocked with G. edulis, U. lactuca and another one as control, without macroalgae and triplicated. The experiment was conducted for two weeks without changing the medium. The purpose of this batch culture was to determine the optimum biofiltration rate that can be supported by the macroalgae at reduced amount of phytoplankton, since the medium has been filtered. The ammonium removal rate without integration of shrimp was also observed.