Establishment of cell suspension culture: The
30 days old callus obtained from leaf disk was used
for establishing cell suspension culture. The 0.5g
callus fresh weight was carefully transferred to
Erlenmyer flask containing 25ml of liquid medium
supplemented with respective callus growth
hormones. The cells were cultured at 125 rpm on
gyratory platform shaker under dark condition at 25
±2 oC for 30 days.