4. Microbial counts
The numeration of viable LAB, yeasts and Enterobacteriaceae in
pickles was determined by plate count method. Pickle juice (1 ml)
sterilely taken from pickle jars was subjected to sequential decimal
dilutions after which 0.1 ml of appropriate decimal dilutions were
spread onto agar media. MRS for LAB was incubated at 37 C for
48 h; potato dextrose agar (PDA) for yeasts was incubated at 25 C
for 48 h; Violet Red Bile Glucose agar (VRBGA, Oxoid England) for
Enterobacteriaceae was incubated at 37 C for 24 h (Li et al., 2015).