There was significant difference in crude fat contents between the traditionally fermented cotton seeds and unfermented cotton seeds as well as cotton seeds fermented under controlled conditions. There was a noticeable decrease in crude fat content in the fermented cotton seeds when compared with the unfermented cotton seeds. This reduction in value was from 28.25% in the unfermented cotton seeds to 15.29% in the fermented cotton seeds using combinations of all the four bacterial cultures in inoculating. This decrease in the crude fat content in the fermented samples could be attributed to their utilisation by the associated microorganisms during fermentation (Sanni and Ogbonna, 1992).