In situ whole cell hybridization and scanning electron microscopic studies of Lumbricus terrestris gut revealed higher bacterial counts in mid-gut region, with bacterial community comprising mainly of unidentified bacteria (Jolly et al. 1993; Fischer et al. 1995). 16S rDNA based clonal surveys of the earthworm gut DNA, denaturing gradient gel electrophoresis and fluorescence in situ hybridization (FISH) using specific oligonucleotide probes are among the modern tools used to study the earthworm gut bacterial diversity. These analyses have documented that representatives of the Acidobacteria, Firmicutes, Proteobacteria, and unclassified group bacteria are tightly