This method is has long been used
for natural dye extraction for a certain of
time. The dye components in dried plant
powder are extracted in water at boil or
particular temperature. Then, the extract is
cooled down and filtered. The dye solution
is carried out under varying conditions as
mentioned above in order to get the
optimal extraction condition. The optimum
condition is determined by studying the
optical density value at definite wavelength
for the extracted solution using UV-Visible
absorbance spectrophotometer (Samanta
and Konar, 2011). The filtrate is further
applied for dyeing. Here are some studies
that used the aqueous extract from plants
for animal tissue staining. Natural dyes were
extracted from kujarat flowers (Hibiscus
sadariffa) in aqueous medium and were
used to replace of eosin stain in kidney from